Extraction and purification of -carotene from filamentous fungus Mucor azygosporus
نویسندگان
چکیده
-carotene has been used for years as natural food colorants and in cosmetics and healthcare due to their antioxidant property. The production of -carotene by Mucor azygosporus was evaluated, utilizing deproteinized waste whey filtrate. Since pigments are intracellular, their extraction needed after cell cultivation. In this study, different strategies for extraction of -carotene from M. azygosporus MTCC 414 were investigated. The combinations of various cell disruption and extraction methods were employed and maximum -carotene (985 μg/ml) was extracted when sonicator was used for disruption of cells, followed by extraction with hexane and ethyl acetate (1:1, v/v). The extracted crude pigment was purified by adsorption column chromatography using neutral alumina. The purified -carotene was, further, analyzed by HPLC before subjecting to NMR and MS.
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